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Mitochondrial Haplotypes Influence Metabolic Traits in Porcine Transmitochondrial Cybrids - Nature.com

... selected by G418 medium with free of uridine and sodium pyruvate. Mitochondria were stained by 1/5000 w/v Janus Green B (Sigma) and nuclei by Giemsa working solution (AppliChem), and all cells were observed under microscopy at ×400 magnification.

Janus Green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis in 1900.

Janus Green B
Janus green B.svg
Ball-and-stick model of the component ions of Janus Green B
Names
IUPAC name
8-(4-Dimethylaminophenyl)diazenyl-N,N-diethyl-10-phenylphenazin-10-ium-2-amine chloride
Other names
Diazin Green S
Union Green B
Identifiers
2869-83-2
3D model (Jmol) Interactive image
ChEBI CHEBI:21184
ChemSpider 68607
ECHA InfoCard 100.018.814
EC Number 220-695-6
PubChem 76123
Properties
C30H31ClN6
Molar mass 511.06 g/mol
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

Janus Green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis in 1900.

The indicator Janus Green B changes colour according to the amount of oxygen present. When oxygen is present, the indicator oxidizes to a blue colour. In the absence of oxygen, the indicator is reduced and changes to a pink colour.

cheek cells janus green

Cheek cells stained with Janus green and viewed with Foldscope.

Janus Green B

Adding Janus Green B dye

For CP Biology, adding Janus Green B dye to celery/sucrose.

Mitochondria with Janus green b

Janus Green B

Janus Green B · Acreil Dissolving Sponge Reveals Wooden Duck ℗ 749945 Records DK Released on: 2017-12-05 Auto-generated by YouTube.

Mitochondria with Janus green b